Hypothermia Affects Translocation of Numerous Cytoplasmic Proteins Following Global Cerebral Ischemia.

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Abstract

Using a decapitation ischemia model, we studied translocation of proteins to and from the cytosol in normothermic (NT) and hypothermic (HT) rat brains. 2D gel analysis identified 74 proteins whose cytosolic level changed significantly after 15 min of ischemia. HT preserved the cytosolic levels of several glycolytic enzymes, as well as many plasticity related proteins, otherwise decreased following NT ischemia. The levels of redox-related proteins was lower in HT than in NT. Our results indicate that translocation of proteins to and from the cytosol is an important issue during ischemia.

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Subject classification (UKÄ) – MANDATORY

  • Other Clinical Medicine

Keywords

  • cytoskeleton, 2D gel cortex, glycolysis, LC-MS/MS, MALDI, plasticity, redox-sensitive
Original languageEnglish
Pages (from-to)2822-2832
JournalJournal of Proteome Research
Volume6
Issue number7
Publication statusPublished - 2007
Publication categoryResearch
Peer-reviewedYes