Hypoxia-inducible factor and vascular endothelial growth factor in the neuroretina and retinal blood vessels after retinal ischemia

Research output: Contribution to journalArticle

Abstract

Retinal ischemia arises from circulatory failure. As the retinal blood vessels are key organs in circulatory failure, our aim was to study the retinal vasculature separately from the neuroretina to elucidate the role of hypoxia-inducible factor (HIF) 1α and 1β and vascular endothelial growth factor (VEGF) in retinal ischemia. Retinal ischemia was induced in porcine eyes by applying an intraocular pressure, followed by 12 h of reperfusion. HIF-1α mRNA expression was not affected by ischemia, while immunofluorescence staining was higher after ischemia in the neuroretina. HIF-1β immu-noreactivity and mRNA expression were unaffected. VEGF protein levels in the vitreous humor and VEGF staining in the neuroretina were more pronounced in eyes subjected to ischemia than in the sham eyes. VEGF may be activated downstream of HIF-1 and is known to stimulate retinal neovascularization, which causes sight-threatening complications. These results emphasize the need for pharmacological treatment to block the HIF and VEGF signaling pathways in retinal ischemia.

Details

Authors
Organisations
External organisations
  • Lund University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Ophthalmology

Keywords

  • Blood vessels, Hypoxia-inducible factor, Ischemia, Porcine, Retina, Vascular endothelial growth factor
Original languageEnglish
Pages (from-to)20-29
Number of pages10
JournalJournal of Ocular Biology, Diseases, and Informatics
Volume3
Issue number1
Publication statusPublished - 2010 Mar
Publication categoryResearch
Peer-reviewedYes

Related activities

Gustafsson, L. (Supervisory team member)
2006 Jan2008 Aug

Activity: Examination and supervisionSupervision of PhD students

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