Hypoxia-inducible factor and vascular endothelial growth factor in the neuroretina and retinal blood vessels after retinal ischemia

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T1 - Hypoxia-inducible factor and vascular endothelial growth factor in the neuroretina and retinal blood vessels after retinal ischemia

AU - Håkansson, Gisela

AU - Gesslein, Bodil

AU - Gustafsson, Lotta

AU - Englund-Johansson, Ulrica

AU - Malmsjö, Malin

PY - 2010/3

Y1 - 2010/3

N2 - Retinal ischemia arises from circulatory failure. As the retinal blood vessels are key organs in circulatory failure, our aim was to study the retinal vasculature separately from the neuroretina to elucidate the role of hypoxia-inducible factor (HIF) 1α and 1β and vascular endothelial growth factor (VEGF) in retinal ischemia. Retinal ischemia was induced in porcine eyes by applying an intraocular pressure, followed by 12 h of reperfusion. HIF-1α mRNA expression was not affected by ischemia, while immunofluorescence staining was higher after ischemia in the neuroretina. HIF-1β immu-noreactivity and mRNA expression were unaffected. VEGF protein levels in the vitreous humor and VEGF staining in the neuroretina were more pronounced in eyes subjected to ischemia than in the sham eyes. VEGF may be activated downstream of HIF-1 and is known to stimulate retinal neovascularization, which causes sight-threatening complications. These results emphasize the need for pharmacological treatment to block the HIF and VEGF signaling pathways in retinal ischemia.

AB - Retinal ischemia arises from circulatory failure. As the retinal blood vessels are key organs in circulatory failure, our aim was to study the retinal vasculature separately from the neuroretina to elucidate the role of hypoxia-inducible factor (HIF) 1α and 1β and vascular endothelial growth factor (VEGF) in retinal ischemia. Retinal ischemia was induced in porcine eyes by applying an intraocular pressure, followed by 12 h of reperfusion. HIF-1α mRNA expression was not affected by ischemia, while immunofluorescence staining was higher after ischemia in the neuroretina. HIF-1β immu-noreactivity and mRNA expression were unaffected. VEGF protein levels in the vitreous humor and VEGF staining in the neuroretina were more pronounced in eyes subjected to ischemia than in the sham eyes. VEGF may be activated downstream of HIF-1 and is known to stimulate retinal neovascularization, which causes sight-threatening complications. These results emphasize the need for pharmacological treatment to block the HIF and VEGF signaling pathways in retinal ischemia.

KW - Blood vessels

KW - Hypoxia-inducible factor

KW - Ischemia

KW - Porcine

KW - Retina

KW - Vascular endothelial growth factor

UR - http://www.scopus.com/inward/record.url?scp=78650213172&partnerID=8YFLogxK

U2 - 10.1007/s12177-010-9050-6

DO - 10.1007/s12177-010-9050-6

M3 - Article

AN - SCOPUS:78650213172

VL - 3

SP - 20

EP - 29

JO - Journal of Ocular Biology, Diseases, and Informatics

JF - Journal of Ocular Biology, Diseases, and Informatics

SN - 1936-8437

IS - 1

ER -