Identification and characterization of secretagogin promoter activity

Research output: Contribution to journalArticle

Abstract

Secretagogin is a newly identified calcium-binding protein selectively expressed in neuroendocrine tissue and pancreatic beta-cells. The function of secretagogin is unknown, but it has been suggested in beta-cells to influence calcium-influx, insulin secretion and proliferation, and has been observed downregulated in diabetes-prone BB rat islets exposed to cytokines. In the present study, we identified and characterized promoter activity of a human 1498 bp sequence upstream the transcription start site. The promoter sequence showed subtle but significant regulation by glucose within the normo-physiological range. Glucose also led to changes in expression of secretagogin protein in INS-1e cells, but not in primary cells from non-diabetes-prone Wistar Furth rats. No effects of cytokines neither on promoter activity nor protein expression were observed. The promoter region was furthermore screened by direct sequencing, and 11 polymorphisms were identified. Genotyping in a large homogenous Type 1 diabetes (T1D) family collection did not reveal association with T1D.

Details

Authors
  • K. V. Skovhus
  • R Bergholdt
  • C Erichsen
  • T. Sparre
  • Jörn Nerup
  • A. E. Karlsen
  • F. Pociot
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area
Original languageEnglish
Pages (from-to)639-645
JournalScandinavian Journal of Immunology
Volume64
Issue number6
Publication statusPublished - 2006
Publication categoryResearch
Peer-reviewedYes