Identification of a novel protein kinase Cδ-Smac complex that dissociates during paclitaxel-induced cell death.

Research output: Contribution to journalArticle

Abstract

Protein kinase C (PKC) δ is a regulator of apoptosis with both pro- and anti-apoptotic effects. The mechanistic basis for the discrepant effects is not completely understood. Here we show that Smac interacts with PKCδ. The interaction depends on the N-terminus of Smac and is disrupted upon treatment with paclitaxel. This is associated with release of Smac into the cytosol. Activation of PKCδ rescues the interaction during paclitaxel exposure and suppresses the paclitaxel-mediated cell death. However, under these conditions the complex is mainly found in the cytosol suggesting that cytosolic Smac can be bound by PKCδ when PKC is activated. The data unravel a previously unrecognized interaction and suggest that PKCδ by associating with Smac may prevent its apoptotic effects. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: PKC deltaphysically interacts with SMAC by anti bait coimmunoprecipitation (View Interaction: 1, 2, 3, 4) XIAPphysically interacts with SMAC by anti tag coimmunoprecipitation(View interaction).

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biological Sciences

Keywords

  • Smac, Protein kinase C, Apoptosis, Cell death, Paclitaxel, XIAP
Original languageEnglish
Pages (from-to)1166-1172
JournalFEBS Letters
Volume586
Issue number8
Publication statusPublished - 2012
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Tumour Cell Biology (013017530)