Identification of Immune Responses Against Aldehyde-Modified Peptide Sequences in ApoB Associated With Cardiovascular Disease.

Research output: Contribution to journalArticle

Abstract

Objective— Atherosclerosis is associated with an immune response against oxidized LDL, which modulates the progression of the disease process.

Methods and Results— Using a library of polypeptides covering the complete sequence of apoB-100, the only major protein of LDL, we have identified over 100 different human antibodies reacting against aldehyde-modified apoB-100 sequences. IgM antibody titer levels decreased with age and were associated with the intima-media thickness of the carotid artery in subjects younger than 60 years. There were also inverse associations between IgM levels and oxidized LDL in plasma. In prospective clinical studies, antibody levels against several aldehyde-modified apoB-100 sites were associated with cardiovascular disease in this age group. Whether this immune response is adaptive (protective) or maladaptive (causal) in atherosclerosis requires further investigation.

Conclusions— We have characterized a large number of epitopes within the apoB-100 component of oxidized LDL that provoke an immune response in humans. These findings will make it possible to study the role of immune responses against specific sites in oxidized LDL and to determine whether these immune responses influence the risk for future cardiac events.

Details

Authors
  • Gunilla Nordin Fredrikson
  • Bo Hedblad
  • Göran Berglund
  • Ragnar Alm
  • Mikko Ares
  • Bojan Cercek
  • Kuang-Yuh Chyu
  • Prediman K. Shah
  • Jan Nilsson
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cardiac and Cardiovascular Systems

Keywords

  • responses, cardiovascular diseases, apolipoproteins, atherosclerosis, peptide sequences, immune
Original languageEnglish
Pages (from-to)872-878
JournalArteriosclerosis, Thrombosis and Vascular Biology
Volume23
Issue number5
Publication statusPublished - 2003
Publication categoryResearch
Peer-reviewedYes