Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells.

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Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells. / Anisimov, Sergey; Christophersen, Nicolaj; Correia, Ana S; Hall, Vanessa; Sandelin, Ingrid; Li, Jia-Yi; Brundin, Patrik.

In: Cellular & Molecular Biology Letters, Vol. 16, No. 1, 2011, p. 79-88.

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Anisimov, Sergey ; Christophersen, Nicolaj ; Correia, Ana S ; Hall, Vanessa ; Sandelin, Ingrid ; Li, Jia-Yi ; Brundin, Patrik. / Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells. In: Cellular & Molecular Biology Letters. 2011 ; Vol. 16, No. 1. pp. 79-88.

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TY - JOUR

T1 - Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells.

AU - Anisimov, Sergey

AU - Christophersen, Nicolaj

AU - Correia, Ana S

AU - Hall, Vanessa

AU - Sandelin, Ingrid

AU - Li, Jia-Yi

AU - Brundin, Patrik

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuronal Survival (013212041), Neural Plasticity and Repair (013210080)

PY - 2011

Y1 - 2011

N2 - The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study identified a limited number of molecular targets potentially involved in the ability of human neonatal foreskin fibroblasts to serve as feeder cells for human embryonic stem cell cultures. Among these, the C-KIT, leptin and pigment epithelium-derived factor (PEDF) genes were the most interesting candidates.

AB - The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study identified a limited number of molecular targets potentially involved in the ability of human neonatal foreskin fibroblasts to serve as feeder cells for human embryonic stem cell cultures. Among these, the C-KIT, leptin and pigment epithelium-derived factor (PEDF) genes were the most interesting candidates.

U2 - 10.2478/s11658-010-0039-8

DO - 10.2478/s11658-010-0039-8

M3 - Article

VL - 16

SP - 79

EP - 88

JO - Cellular and Molecular Biology Letters

T2 - Cellular and Molecular Biology Letters

JF - Cellular and Molecular Biology Letters

SN - 1425-8153

IS - 1

ER -