Identification of non-heme diiron proteins that catalyze triple bond and epoxy group formation

Research output: Contribution to journalArticle

Abstract

Acetylenic bonds are present in more than 600 naturally occurring compounds. Plant enzymes that catalyze the formation of the Delta12 acetylenic bond in 9-octadecen-12-ynoic acid and the Delta12 epoxy group in 12,13-epoxy-9-octadecenoic acid were characterized, and two genes, similar in sequence, were cloned. When these complementary DNAs were expressed in Arabidopsis thaliana, the content of acetylenic or epoxidated fatty acids in the seeds increased from 0 to 25 or 15 percent, respectively. Both enzymes have characteristics similar to the membrane proteins containing non-heme iron that have histidine-rich motifs.

Details

Authors
  • M Lee
  • Marit Lenman
  • A Banas
  • M Bafor
  • S Singh
  • M Schweizer
  • R Nilsson
  • C Liljenberg
  • A Dahlqvist
  • PO Gummeson
  • S Sjödahl
  • A Green
  • S Stymne
External organisations
  • External Organization - Unknown
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biological Sciences
Original languageEnglish
Pages (from-to)915-918
JournalScience
Volume280
Issue number5365
Publication statusPublished - 1998
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Cell and Organism Biology (Closed 2011.) (011002100)