Identification of non-heme diiron proteins that catalyze triple bond and epoxy group formation
Research output: Contribution to journal › Article
Abstract
Acetylenic bonds are present in more than 600 naturally occurring compounds. Plant enzymes that catalyze the formation of the Delta12 acetylenic bond in 9-octadecen-12-ynoic acid and the Delta12 epoxy group in 12,13-epoxy-9-octadecenoic acid were characterized, and two genes, similar in sequence, were cloned. When these complementary DNAs were expressed in Arabidopsis thaliana, the content of acetylenic or epoxidated fatty acids in the seeds increased from 0 to 25 or 15 percent, respectively. Both enzymes have characteristics similar to the membrane proteins containing non-heme iron that have histidine-rich motifs.
Details
| Authors | |
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| External organisations |
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| Research areas and keywords | Subject classification (UKÄ) – MANDATORY
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| Original language | English |
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| Pages (from-to) | 915-918 |
| Journal | Science |
| Volume | 280 |
| Issue number | 5365 |
| Publication status | Published - 1998 |
| Publication category | Research |
| Peer-reviewed | Yes |
| Externally published | Yes |
Bibliographic note
The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Department of Cell and Organism Biology (Closed 2011.) (011002100)
