Identification of novel immunogenic proteins of Helicobacter pylori by proteome technology.

Research output: Contribution to journalArticle

Abstract

Cell surface proteins of the human gastric pathogen Helicobacter pylori, reference strain CCUG 17874, were extracted with acid glycine and fractionated by heparin affinity chromatography. The extracts were subsequently analysed using high-resolution two-dimensional gel electrophoresis (2-DE) and immunoblotting. Four proteins of low molecular masses (25-30 kDa) stained by Coomassie R-350, were identified by peptide ESI-MS/MS sequencing after in-gel tryptic digestion. The identified proteins were recognised by sera from H. pylori-infected patients. Two of them are now described for the first time as immunogenic proteins of which one protein was determined to be distinct from all H. pylori proteins previously described. In addition, the specificity of the identified peptides was evaluated using both 1-D and 2-D immunoblotting against a panel of sera from patients with various bacterial infections. The present identification of highly specific antigens of H. pylori will encourage the improvement of serological diagnostic tests to diagnose and monitor H. pylori infection.

Details

Authors
  • Meeme Utt
  • Ingrid Nilsson
  • Åsa Ljungh
  • Torkel Wadström
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area

Keywords

  • Two-Dimensional, Helicobacter Infections, Gel, Bacterial Outer Membrane Proteins, Electrophoresis, Proteome, Serologic Tests, Antigens, Immunoblotting
Original languageEnglish
Pages (from-to)1-10
JournalJournal of Immunological Methods
Volume259
Issue number1-2
Publication statusPublished - 2002
Publication categoryResearch
Peer-reviewedYes