Identification of the minimal glycopeptide core recognized by T cells in a model for rheumatoid arthritis
Research output: Contribution to journal › Article
Abstract
Collagen induced arthritis (CIA) is a common mouse model for rheumatoid arthritis. Two sets of truncated peptides derived from type II collagen have been prepared and tested for binding to A(q), a MHC-II molecule associated with development of CIA. Binding to A(q) correlated well with predictions from a computer-based model. T-cell hybridomas, obtained in CIA, were also used to study the ability of A(q) bound peptides to trigger a T-cell response. The minimal peptide epitope required for binding, as well as for giving a T-cell response, was determined to be CII260-267. In collagen this epitope is often glycosylated at hydroxylysine 264 and glycosylation has been shown to be an immunodominant feature in CIA. Synthesis and evaluation of CII260-267 carrying a beta-D-galactosyl moiety at position 264 revealed that this glycopeptide stimulated representative members from a panel of carbohydrate-specific T-cell hybridomas obtained in CIA.
Details
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Organisations | |
Research areas and keywords | Subject classification (UKÄ) – MANDATORY
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Original language | English |
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Pages (from-to) | 473-482 |
Journal | Bioorganic & Medicinal Chemistry |
Volume | 13 |
Issue number | 2 |
Publication status | Published - 2005 |
Publication category | Research |
Peer-reviewed | Yes |
Bibliographic note
The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Medical Inflammation Research (013212019)