Identification of two C-terminal autophosphorylation sites in the PDGF beta-receptor: involvement in the interaction with phospholipase C-gamma

Research output: Contribution to journalArticle

Abstract

Two novel sites of autophosphorylation were localized to the C-terminal tail of the PDGF beta-receptor. To evaluate the importance of these phosphorylation sites, receptor mutants in which Tyr1009, Tyr1021 or both were replaced with phenylalanine residues, were expressed in porcine aortic endothelial (PAE) cells. These mutants were similar to the wild type receptor with regard to protein tyrosine kinase activity and ability to induce mitogenicity in response to PDGF-BB. However, both the Y1009F and Y1021F mutants showed a decreased ability to mediate association with and the tyrosine phosphorylation of phospholipase C-gamma (PLC-gamma) compared to the wild type PDGF beta-receptor; in the case of the Y1009F/Y1021F double mutant, no association or phosphorylation of PLC-gamma could be detected. These data show that tyrosine phosphorylation of PLC-gamma is dependent on autophosphorylation of the PDGF beta-receptor at Tyr1009 and Tyr1021.

Details

Authors
  • Lars Rönnstrand
  • Seijiro Mori
  • Ann-Kristin Arvidsson
  • Anders Eriksson
  • Christer Wernstedt
  • Ulf Hellman
  • Lena Claesson-Welsh
  • Carl-Henrik Heldin
External organisations
  • External Organization - Unknown
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medicinal Chemistry

Keywords

  • Amino Acid Sequence Animals Base Sequence Cell Line Female Isoenzymes/*metabolism Kinetics Macromolecular Substances Models, Site-Directed Oligodeoxyribonucleotides Peptide Fragments/isolation & purification Peptide Mapping Phosphates/*metabolism Phosphopeptides/isolation & purification Phosphorylation Plasmids Platelet-Derived Growth Factor/*metabolism Receptors, Structural Molecular Sequence Data Mutagenesis, Platelet-Derived Growth Factor/genetics/*metabolism Recombinant Proteins/metabolism Swine Transfection Type C Phospholipases/*metabolism Uterus/metabolism
Original languageEnglish
Pages (from-to)3911-3919
JournalEMBO Journal
Volume11
Issue number11
Publication statusPublished - 1992
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Experimental Clinical Chemistry (013016010)