Identification of Tyr-703 and Tyr-936 as the primary association sites for Grb2 and Grb7 in the c-Kit/stem cell factor receptor

Research output: Contribution to journalArticle

Abstract

In this paper we demonstrate the presence of two novel in vivo autophosphorylation sites in the c-Kit/stem cell factor receptor (c-Kit/SCFR): Tyr-703 in the kinase insert and Tyr-936 in the C-terminal tail. We furthermore demonstrate that the adapter protein Grb2 is a specific binding partner for both phosphorylated Tyr-703 and phosphorylated Tyr-936, whereas the adapter protein Grb7 binds selectively to phosphorylated Tyr-936. It is shown that the association occurs through the Src homology 2 (SH2) domains of Grb2 and Grb7. Binding of Grb2 to Tyr-703 in the c-Kit/SCFR provides a link to the Ras/mitogen-activated protein kinase pathway.

Details

Authors
External organisations
  • External Organization - Unknown
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medicinal Chemistry

Keywords

  • Signal Transducing Cells, *Adaptor Proteins, Cultured Endothelium, Vascular/cytology GRB2 Adaptor Protein GRB7 Adaptor Protein Humans Phosphorylation Protein Binding Proteins/*metabolism Proto-Oncogene Proteins c-kit/genetics/*metabolism Receptor Protein-Tyrosine Kinases/genetics/metabolism Recombinant Fusion Proteins/metabolism Stem Cell Factor *Tyrosine src Homology Domains
Original languageEnglish
Pages (from-to)211-216
JournalBiochemical Journal
Volume341
Issue number1
Publication statusPublished - 1999
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Experimental Clinical Chemistry (013016010)