Identifying bacteria using DNA binding maps

Research output: Chapter in Book/Report/Conference proceedingPaper in conference proceeding

Abstract

We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known sequence. The goal is to create a method for fast identification of bacteria from single DNA molecules without the need for additional cultivation or amplification. We here demonstrate a proof-of-principle experiment on phage DNA and furthermore show that the method can be used to distinguish between two strains of E. coli DNA and to map pieces of DNA onto the full genome.

Details

Authors
Organisations
External organisations
  • Chalmers University of Technology
  • Sahlgrenska Academy
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biophysics

Keywords

  • Bacterial identification, Nanofluidics, Optical mapping
Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages473-475
Number of pages3
Volume1
ISBN (Print)9781632666246
Publication statusPublished - 2013 Jan 1
Publication categoryResearch
Peer-reviewedYes
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period2013/10/272013/10/31