Imaging apolipoprotein AI in vivo

Research output: Contribution to journalArticle

Abstract

Coronary disease risk increases inversely with high-density lipoprotein (HDL) level. The measurement of the biodistribution and clearance of HDL in vivo, however, has posed a technical challenge. This study presents an approach to the development of a lipoprotein MRI agent by linking gadolinium methanethiosulfonate (Gd[MTS-ADO3A]) to a selective cysteine mutation in position 55 of apo AI, the major protein of HDL. The contrast agent targets both liver and kidney, the sites of HDL catabolism, whereas the standard MRI contrast agent, gadolinium-diethylenetriaminepentaacetic acid-bismethylamide (GdDTPA-BMA, gadodiamide), enhances only the kidney image. Using a modified apolipoprotein AI to create an HDL contrast agent provides a new approach to investigate HDL biodistribution, metabolism and regulation in vivo. Copyright (C) 2011 John Wiley & Sons, Ltd.

Details

Authors
  • Renuka Sriram
  • Jens Lagerstedt
  • Jitka Petrlova
  • Haris Samardzic
  • Ulrike Kreutzer
  • Hongtao Xie
  • George A. Kaysen
  • Jean F. Desreux
  • David Thonon
  • Vincent Jacques
  • Martha Van Loan
  • John C. Rutledge
  • Michael N. Oda
  • John C. Voss
  • Thomas Jue
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Radiology, Nuclear Medicine and Medical Imaging

Keywords

  • NMR, apolipoprotein, HDL, contrast agent, MRI, gadolinium, cardiovascular risk
Original languageEnglish
Pages (from-to)916-924
JournalNMR in Biomedicine
Volume24
Issue number7
Publication statusPublished - 2011
Publication categoryResearch
Peer-reviewedYes