Immunoglobulin from insulin-dependent diabetic children inhibits glucose-induced insulin release

Research output: Contribution to journalArticle

Abstract

Immunoglobulin was separated from islet cel antibody positive plasma of six children with newly diagnosed insulin-dependent (type I) diabetes mellitus. The dynamics of insulin release in response to glucose and partially purified antibodies were determined in dispersed rat islet cells perifused on small columns of Biogel P-2 beads. After perifusion at 5.5 mmol/L D-glucose in the presence of healthy control immunoglobulin, the rate of insulin release increased in a biphasic manner after stimulation with 30 mmol/L D-glucose. In cells exposed to diabetic immunoglobulin, 30 mmol/L D-glucose had little, if any, effect on insulin release. These results suggest that islet cell antibodies in insulin-dependent diabetes may interfere with the insulin release mechanisms in the pancreatic B-cells.

Details

Authors
External organisations
  • Hagedorn Research Institute
Original languageEnglish
Pages (from-to)520-524
Number of pages5
JournalDiabetes
Volume32
Issue number6 I
Publication statusPublished - 1983 Jan 1
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes