Immunoglobulin subclass profiles of anti-idiotypic antibodies to GAD65Ab differ between type 1 diabetes patients and healthy individuals.

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Abstract

Previously we reported the presence of anti-idiotypic antibodies (anti-Id) specific to autoantibodies against GAD65 (GAD65Ab) in healthy individuals while the activity of anti-Id directed to GAD65Ab in T1D patients was significantly lower. These anti-Id recognize the antigen binding site of GAD65Ab, thus preventing their binding to GAD65. Here we characterized the IgG subclass profile of these anti-Id (GAD65Ab-specific) and of the associated GAD65Ab themselves. The IgG subclass response of anti-Id in healthy individuals (n=16) was IgG3-dominated, while in T1D patients (n=8) IgG1 was the major IgG subclass. The GAD65Ab bound by anti-Id in both healthy individuals (n=38) and GAD65Ab-negative T1D patients (n=35) showed a predominant rank order of IgG1>IgG2>IgG4>IgG3. However, the frequency of GAD65Ab of the IgG4 subclass was significantly higher in T1D patients (p<0.05). We conclude that the IgG subclass profile of anti-Id (GAD65Ab-specific) in healthy individuals differs from that in T1D patients. These differences may provide insights in the development of these antibodies.

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  • Immunology in the medical area
Original languageEnglish
Pages (from-to)363-367
JournalScandinavian Journal of Immunology
Volume74
Publication statusPublished - 2011
Publication categoryResearch
Peer-reviewedYes