Immunohistochemical studies on the distribution of albumin, fibrinogen, fibronectin, IgG and collagen around PTFE and titanium implants

Research output: Contribution to journalArticle


Time-dependent distribution of extracellular proteins (albumin, fibrinogen, fibronectin, collagen-I and IgG) in the interface zone between implant and soft tissue has been investigated utilizing a recently developed method. Commercially pure (c.p.) titanium and polytetrafluoroethylene (PTFE) implants were inserted in the abdominal wall of rats for 1, 6 and 12 weeks followed by a mild fixation, cryoprotection, rapid freezing in LN2-cooled propane, cryosubstitution and low-temperature infiltration with UV curing of the methacrylate LR-Gold. Before sectioning, the bulk part of the titanium was removed by an electrolytical dissolution technique (electropolishing), while the PTFE implants were removed by a fracture technique. Employing a cryosubstitution method combined with postembedding immunohistochemistry, a light microscopic analysis was allowed. The selected proteins had an apparently varying distribution in the implant-close tissue and their distribution changed during the follow-up period. There was also a difference in the distribution pattern for each protein around titanium and PTFE implants. Insertion of the c.p. titanium implants elicited an inflammatory reaction in many respects similar to a normal wound healing response, while the PTFE implants caused a more pronounced, persistent inflammation.


  • Agneta Rosengren
  • B R Johansson
  • N Danielsen
  • P. Thomsen
  • L E Ericson
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Basic Medicine


  • Abdominal Muscles, Animals, Biocompatible Materials, Collagen, Fibrinogen, Fibronectins, Immunoglobulin G, Immunohistochemistry, Male, Muscle Fibers, Skeletal, Polytetrafluoroethylene, Prostheses and Implants, Rats, Rats, Sprague-Dawley, Serum Albumin, Titanium, Journal Article, Research Support, Non-U.S. Gov't
Original languageEnglish
Pages (from-to)1779-86
Number of pages8
Issue number18
Publication statusPublished - 1996 Sep
Publication categoryResearch