Immunophenotypical characterization of human neutrophil differentiation

Research output: Contribution to journalArticle

Abstract

The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs. J. Leukoc. Biol. 90: 629-634; 2011.

Details

Authors
  • Helena Mora-Jensen
  • Johan Jendholm
  • Anna Fossum
  • Bo Porse
  • Niels Borregaad
  • Kim Theilgaard-Moench
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology

Keywords

  • granulocytes, myeloid differentiation, differentiation pathway, flow, cytometry, human bone marrow
Original languageEnglish
Pages (from-to)629-634
JournalJournal of Leukocyte Biology
Volume90
Issue number3
Publication statusPublished - 2011
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hematology/Transplantation (013022014), Stem Cell Center (013022011)