Impact of DNA polymerases and their buffer systems on quantitative real-time PCR

Research output: Contribution to journalArticle

Abstract

An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.

Details

Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Microbiology in the medical area
Original languageEnglish
Pages (from-to)408-411
JournalJournal of Clinical Microbiology
Volume42
Issue number1
Publication statusPublished - 2004
Publication categoryResearch
Peer-reviewedYes

Total downloads

No data available