Impact of molecular linker size on physicochemical properties of assembled gold nanoparticle mono-/multi-layers and their applicability for functional binding of biomolecules

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Bibtex

@article{c4d67419c54749178f4e50af69ec1e82,
title = "Impact of molecular linker size on physicochemical properties of assembled gold nanoparticle mono-/multi-layers and their applicability for functional binding of biomolecules",
abstract = "In this work the impact of molecular inter-linker size on gold nanoparticle (AuNP) mono-/multilayer structural properties, density and homogeneity has been investigated. These characteristics are of great importance for functional binding of biomolecules. Positively charged high or low molecular weight inter-linkers, poly-L-lysine (PLL) or N-(6-mercapto)hexylpyridinium (MHP), were used to attach negatively charged AuNPs on a planar gold surface as well as to further interlink into a multilayer structure via layer-by-layer deposition. The inter-particle interaction within the assembled AuNP films was adjusted by the ionic strength in the AuNPs dispersions. The AuNP layer density and structural/viscoelastic properties were evaluated by the quartz crystal microbalance with dissipation (QCM-D) technique. The validity of the commercial Voigt model, specifically developed for quantitative QCM-D data analysis of homogeneous viscoelastic films, was evaluated by a model independent analysis when comparing the assembled AuNP films with a homogeneous layer of a mucin from bovine submaxillary glands. Both AuNP mono- and multilayers, attached/interlinked via long flexible PLL molecules assembled to denser and more soft/viscous structures compared to those interlinked by short MHP compounds. Thus, PLL-interlinked AuNP mono-/multilayer structures were further investigated as a platform for laccase enzyme functional adsorption via qualitative assessment of bioelectrochemical characteristics of the enzyme.",
keywords = "Bio-electro-catalysis, Biomolecule adsorption, Electrochemical-quartz crystal microbalance with dissipation, Gold nanoparticles, Layer-by-layer deposition, N-(6-mercaptohexyl)pyridinium, Poly-L-lysine",
author = "Vida Krikstolaityte and Jildiz Hamit-Eminovski and Laura Abariute and Gediminas Niaura and Rolandas Meskys and Thomas Arnebrant and Grzegorz Lisak and Tautgirdas Ruzgas",
year = "2019",
doi = "10.1016/j.jcis.2019.02.048",
language = "English",
volume = "543",
pages = "307--316",
journal = "Journal of Colloid and Interface Science",
issn = "0021-9797",
publisher = "Elsevier",

}