Impact of molecular linker size on physicochemical properties of assembled gold nanoparticle mono-/multi-layers and their applicability for functional binding of biomolecules

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Impact of molecular linker size on physicochemical properties of assembled gold nanoparticle mono-/multi-layers and their applicability for functional binding of biomolecules. / Krikstolaityte, Vida; Hamit-Eminovski, Jildiz; Abariute, Laura; Niaura, Gediminas; Meskys, Rolandas; Arnebrant, Thomas; Lisak, Grzegorz; Ruzgas, Tautgirdas.

In: Journal of Colloid and Interface Science, Vol. 543, 2019, p. 307-316.

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Krikstolaityte, Vida ; Hamit-Eminovski, Jildiz ; Abariute, Laura ; Niaura, Gediminas ; Meskys, Rolandas ; Arnebrant, Thomas ; Lisak, Grzegorz ; Ruzgas, Tautgirdas. / Impact of molecular linker size on physicochemical properties of assembled gold nanoparticle mono-/multi-layers and their applicability for functional binding of biomolecules. In: Journal of Colloid and Interface Science. 2019 ; Vol. 543. pp. 307-316.

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TY - JOUR

T1 - Impact of molecular linker size on physicochemical properties of assembled gold nanoparticle mono-/multi-layers and their applicability for functional binding of biomolecules

AU - Krikstolaityte, Vida

AU - Hamit-Eminovski, Jildiz

AU - Abariute, Laura

AU - Niaura, Gediminas

AU - Meskys, Rolandas

AU - Arnebrant, Thomas

AU - Lisak, Grzegorz

AU - Ruzgas, Tautgirdas

PY - 2019

Y1 - 2019

N2 - In this work the impact of molecular inter-linker size on gold nanoparticle (AuNP) mono-/multilayer structural properties, density and homogeneity has been investigated. These characteristics are of great importance for functional binding of biomolecules. Positively charged high or low molecular weight inter-linkers, poly-L-lysine (PLL) or N-(6-mercapto)hexylpyridinium (MHP), were used to attach negatively charged AuNPs on a planar gold surface as well as to further interlink into a multilayer structure via layer-by-layer deposition. The inter-particle interaction within the assembled AuNP films was adjusted by the ionic strength in the AuNPs dispersions. The AuNP layer density and structural/viscoelastic properties were evaluated by the quartz crystal microbalance with dissipation (QCM-D) technique. The validity of the commercial Voigt model, specifically developed for quantitative QCM-D data analysis of homogeneous viscoelastic films, was evaluated by a model independent analysis when comparing the assembled AuNP films with a homogeneous layer of a mucin from bovine submaxillary glands. Both AuNP mono- and multilayers, attached/interlinked via long flexible PLL molecules assembled to denser and more soft/viscous structures compared to those interlinked by short MHP compounds. Thus, PLL-interlinked AuNP mono-/multilayer structures were further investigated as a platform for laccase enzyme functional adsorption via qualitative assessment of bioelectrochemical characteristics of the enzyme.

AB - In this work the impact of molecular inter-linker size on gold nanoparticle (AuNP) mono-/multilayer structural properties, density and homogeneity has been investigated. These characteristics are of great importance for functional binding of biomolecules. Positively charged high or low molecular weight inter-linkers, poly-L-lysine (PLL) or N-(6-mercapto)hexylpyridinium (MHP), were used to attach negatively charged AuNPs on a planar gold surface as well as to further interlink into a multilayer structure via layer-by-layer deposition. The inter-particle interaction within the assembled AuNP films was adjusted by the ionic strength in the AuNPs dispersions. The AuNP layer density and structural/viscoelastic properties were evaluated by the quartz crystal microbalance with dissipation (QCM-D) technique. The validity of the commercial Voigt model, specifically developed for quantitative QCM-D data analysis of homogeneous viscoelastic films, was evaluated by a model independent analysis when comparing the assembled AuNP films with a homogeneous layer of a mucin from bovine submaxillary glands. Both AuNP mono- and multilayers, attached/interlinked via long flexible PLL molecules assembled to denser and more soft/viscous structures compared to those interlinked by short MHP compounds. Thus, PLL-interlinked AuNP mono-/multilayer structures were further investigated as a platform for laccase enzyme functional adsorption via qualitative assessment of bioelectrochemical characteristics of the enzyme.

KW - Bio-electro-catalysis

KW - Biomolecule adsorption

KW - Electrochemical-quartz crystal microbalance with dissipation

KW - Gold nanoparticles

KW - Layer-by-layer deposition

KW - N-(6-mercaptohexyl)pyridinium

KW - Poly-L-lysine

U2 - 10.1016/j.jcis.2019.02.048

DO - 10.1016/j.jcis.2019.02.048

M3 - Article

VL - 543

SP - 307

EP - 316

JO - Journal of Colloid and Interface Science

T2 - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

SN - 0021-9797

ER -