Improved MALDI-TOF-MS analysis of partially depolymerised methyl cellulose by reducing end derivatisation

Research output: Chapter in Book/Report/Conference proceedingPaper in conference proceeding

Abstract

The depolymerized methyl cellulose was analyzed, using improved MALDI-TOF-MS by reducing the end derivatization. The methyl cellulose was depolymerized to give methylated oligo-(1->4)-β-D-anhydroglucoses (M-oligo-AG). To reduce the errors during measurement of degree of substitution (DS), M-oligo-AG was derivatized with Girard's T reagent performed on an equimolar mixture of maltooligosaccharides. The GT-derivatized M-oligo-AG (GT-M-oligo-AG) formed singly charged molecular ions with peaks at m/z values 91 higher than the corresponding peaks of M-oligo-AG. The results show that for GT-M-oligo-AG the relative signal intensities for DP=1 and 2 were increased significantly.

Details

Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Analytical Chemistry

Keywords

  • Methyl cellulose, Matrix-assisted laser desorption/ionization (MALDI), Time-of flight (TOF) mass spectrometry, Maltooligosaccharides
Original languageEnglish
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrmetry and Allied Topics
PublisherAmerican Society for Mass Spectrometry, Santa Fe, NM, 87505, United States
Pages421-422
Publication statusPublished - 2002
Publication categoryResearch
Peer-reviewedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2002 Jun 22002 Jun 6

Conference

ConferenceProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/TerritoryUnited States
CityOrlando, FL
Period2002/06/022002/06/06

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)