Improved positioning and detectability of microparticles in droplet microfluidics using two-dimensional acoustophoresis

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Improved positioning and detectability of microparticles in droplet microfluidics using two-dimensional acoustophoresis. / Ohlin, M.; Fornell, A.; Bruus, H.; Tenje, M.

In: Journal of Micromechanics and Microengineering, Vol. 27, No. 8, 084002, 20.07.2017.

Research output: Contribution to journalArticle

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TY - JOUR

T1 - Improved positioning and detectability of microparticles in droplet microfluidics using two-dimensional acoustophoresis

AU - Ohlin, M.

AU - Fornell, A.

AU - Bruus, H.

AU - Tenje, M.

PY - 2017/7/20

Y1 - 2017/7/20

N2 - We have fabricated a silicon-glass two-phase droplet microfluidic system capable of generating sub 100 μm-sized, ø = (74 ± 2) μm, spherical droplets at rates of up to hundreds of hertz. By implementing a two-dimensional (2D) acoustophoresis particle-positioning method, we show a fourfold improvement in both vertical and lateral particle positioning inside the droplets compared to unactuated operation. The efficiency of the system has been optimized by incorporating aluminum matching layers in the transducer design permitting biocompatible operational temperatures (<37 °C). Furthermore, by using acoustic actuation, (99.8 ± 0.4)% of all encapsulated microparticles can be detected compared to only (79.0 ± 5.1)% for unactuated operation. In our experiments we observed a strong ordering of the microparticles in distinct patterns within the droplet when using 2D acoustophoresis; to explain the origin of these patterns we simulated numerically the fluid flow inside the droplets and compared with the experimental findings.

AB - We have fabricated a silicon-glass two-phase droplet microfluidic system capable of generating sub 100 μm-sized, ø = (74 ± 2) μm, spherical droplets at rates of up to hundreds of hertz. By implementing a two-dimensional (2D) acoustophoresis particle-positioning method, we show a fourfold improvement in both vertical and lateral particle positioning inside the droplets compared to unactuated operation. The efficiency of the system has been optimized by incorporating aluminum matching layers in the transducer design permitting biocompatible operational temperatures (<37 °C). Furthermore, by using acoustic actuation, (99.8 ± 0.4)% of all encapsulated microparticles can be detected compared to only (79.0 ± 5.1)% for unactuated operation. In our experiments we observed a strong ordering of the microparticles in distinct patterns within the droplet when using 2D acoustophoresis; to explain the origin of these patterns we simulated numerically the fluid flow inside the droplets and compared with the experimental findings.

KW - acoustophoresis

KW - droplet microfluidics

KW - microparticle detection

KW - microparticle manipulation

KW - ultrasonic standing wave

U2 - 10.1088/1361-6439/aa7967

DO - 10.1088/1361-6439/aa7967

M3 - Article

VL - 27

JO - Journal of Micromechanics and Microengineering

T2 - Journal of Micromechanics and Microengineering

JF - Journal of Micromechanics and Microengineering

SN - 0960-1317

IS - 8

M1 - 084002

ER -