Improvement of retroviral retargeting by using amino acid spacers between an additional binding domain and the N terminus of Moloney murine leukemia virus SU

Research output: Contribution to journalArticle

Abstract

We previously reported a strategy to redirect the retroviral host range by expressing single-chain antibodies (S. J. Russell, R. E. Hawkins, and G. Winter, Nucleic Acids Res. 21:1081-1085, 1993) or ligands (F.-L. Cosset, F. J Morling, Y. Takeuchi, R. A. Weiss, M. K. L. Collins, and S. J. Russell, J. Virol. 69:6314-6322, 1995) at the N terminus of Moloney murine leukemia virus (MoMLV) surface proteins (SU). Although such chimeric envelopes were able to bind the new receptors, the transduction efficiency of retargeted viruses was generally low. We hypothesized that conformational rearrangements of envelope glycoproteins were not optimally triggered following binding, and to overcome these postbinding blocks, we have generated here a set of chimeric MoMLV-derived envelopes targeted to the Ram-1 phosphate transporter in which we have varied the spacing between the Ram-1-binding domain and the MoMLV SU. All of the recombinant envelopes were correctly expressed on virions, and all bound efficiently to Ram-1. However, the interdomain spacing greatly affected the efficiency of gene transfer by retroviral vectors that had bound to Ram-1 via their chimeric envelopes. Optimal interdomain spacing allowed a 100-fold-increased viral transduction via Ram-1 compared to our previous results.

Details

Authors
  • S Valsesia-Wittmann
  • F J Morling
  • B H Nilson
  • Y Takeuchi
  • S J Russell
  • F L Cosset
External organisations
  • Claude Bernard University Lyon 1
  • Cambridge Centre for Protein Engineering, MRC Centre
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Basic Medicine

Keywords

  • 3T3 Cells, Animals, Base Sequence, Cell Line, Cloning, Molecular, DNA, Viral, Humans, Mice, Molecular Sequence Data, Moloney murine leukemia virus, Mutagenesis, Phosphate Transport Proteins, Receptors, Virus, Recombinant Fusion Proteins, Retroviridae Proteins, Oncogenic, Sodium-Phosphate Cotransporter Proteins, Sodium-Phosphate Cotransporter Proteins, Type III, Symporters, Transformation, Genetic, Viral Envelope Proteins, Journal Article, Research Support, Non-U.S. Gov't
Original languageEnglish
Pages (from-to)2059-2064
Number of pages6
JournalJournal of Virology
Volume70
Issue number3
Publication statusPublished - 1996 Mar
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes