In vivo electrochemical studies of optogenetic control of glutamate signaling measured using enzyme-based ceramic microelectrode arrays

Research output: Chapter in Book/Report/Conference proceedingBook chapter

Abstract

Direct electrochemical measurements of glutamate release in vivo were combined with optogenetics in order to examine light-induced control of glutamate neurotransmission in the rodent brain. Self-referenced recordings of glutamate using ceramic-based microelectrode arrays (MEAs) in hippocampus and frontal cortex demonstrated precise optical control of light-induced glutamate release through channelrhodopsin (ChR2) expression in both rat hippocampus and frontal cortex. Although the virus was only injected unilaterally, bilateral and rostro-caudal expression was observed in slice imaging, indicating diffusion and active transport of the viral particles. Methodology for the optogenetic control of glutamate signaling in the rat brain is thoroughly explained with special attention paid to MEA enzyme coating and cleaning for the benefit of other investigators. These data support that optogenetic control of glutamate signaling is robust with certain advantages as compared to other methods to modulate the in vivo control of glutamate signaling.

Details

Authors
  • Jason J. Burmeister
  • Francois Pomerleau
  • Jorge E. Quintero
  • Peter Huettl
  • Yi Ai
  • Johan Jakobsson
  • Martin Lundblad
  • Andreas Heuer
  • John T Slevin
  • Greg A. Gerhardt
Organisations
External organisations
  • University of Kentucky Medical Center
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Neurosciences

Keywords

  • Amperometry, Array, Biosensor, Electrochemistry, Glutamate, Glutamate oxidase, Microelectrode, Neurotransmitter, Optogenetics
Original languageEnglish
Title of host publicationNeuromethods
PublisherHumana Press
Pages327-351
Number of pages25
Volume130
Publication statusPublished - 2018
Publication categoryResearch
Peer-reviewedYes

Publication series

NameNeuromethods
Volume130
ISSN (Print)08932336
ISSN (Electronic)19406045