Increased GABA(A) channel subunits expression in CD8(+) but not in CD4(+) T cells in BB rats developing diabetes compared to their congenic littermates

Research output: Contribution to journalArticle

Abstract

GABA (gamma-aminobutyric acid), the main inhibitory neurotransmitter in the central nervous system is also present in the pancreatic islet beta cells where it may function as a paracrine molecule and perhaps as an immunomodulator of lymphocytes infiltrating the pancreatic islet. We examined CD4(+) and CD8(+) T cells from diabetes prone (DRlyp/lyp) or resistant (DR+/+) congenic biobreeding (BB) rats for expression of GABA(A) channels. Our results show that BB rat CD4(+) and CD8(+) T cells express alpha 1, alpha 2, alpha 3, alpha 4, alpha 6, beta 3, gamma 1, delta, rho 1 and rho 2 CABA(A) channel subunits. In CD8(+) T cells from DRlyp/lyp animals the subunits were significantly upregulated relative to expression levels in the CD8+ T cells from DR+/+ rats as well as from CD4(+) T cells from both DRlyp/lyp and DR+/+ rats. Functional channels were formed in the T cells and physiological concentrations of GABA (100 nM) decreased T cell proliferation. Our results are consistent with the hypothesis that GABA in the islets of Langerhans may diminish inflammation by inhibition of activated T lymphocytes. (C) 2010 Elsevier Ltd. All rights reserved.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area

Keywords

  • GABA, Diabetes, Lymphocytes, GABA(A) subunits, Proliferation, Immunomodulation
Original languageEnglish
Pages (from-to)399-407
JournalMolecular Immunology
Volume48
Issue number4
Publication statusPublished - 2011
Publication categoryResearch
Peer-reviewedYes

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