Induced N-limitation of bacterial growth in soil: Effect of carbon loading and N status in soil

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Induced N-limitation of bacterial growth in soil: Effect of carbon loading and N status in soil. / Kamble, Pramod N.; Bååth, Erland.

In: Soil Biology & Biochemistry, Vol. 74, 2014, p. 11-20.

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TY - JOUR

T1 - Induced N-limitation of bacterial growth in soil: Effect of carbon loading and N status in soil

AU - Kamble, Pramod N.

AU - Bååth, Erland

PY - 2014

Y1 - 2014

N2 - Application of C-rich plant residues can change the soil system from C-limitation for microbial growth to limitation by other nutrients. However, the initial nutrient status of the soil may interact with the added amount of residues in determining limitation. We studied this interactive effect in soils from the Harvard Forest LTER, where annual addition of N since 1988 has resulted in soils with different N-status: No N (Unfertilized), 50 (Low N) and 150 (High N) kg N ha(-1). We hypothesized that adding C-rich substrate would change the soil from being C- to being N-limited for bacterial growth and that the extent of Nlimitation would be higher with increasing substrate additions, while becoming less evident in soil with increasing N-status. We compared the effect of adding two C-rich substrates, starch (0, 10, 20, 40 mg g(-1) soil) and straw (0, 20, 40, 80 mg g(-1)), incubating the soils for up to 3 and 4 weeks for starch and straw, respectively. Nutrient limitations were studied by measuring bacterial growth 3 days after adding C as glucose and N as NH4NO3 in a full factorial design. Initially bacterial growth in all soils was C-limited. As hypothesized, adding C-rich substrates removed the C-limitation, with lower amounts of starch and straw needed in the unfertilized and Low N soils than in the High N soil. Combinations of different Nstatus of the soil and amendment levels of starch and straw could be found, where bacterial growth appeared close to co-limited both by available C and N. However, at even higher amendment levels, presumable resulting in N-limitation, bacterial growth still responded less by adding N then C-limited soils by adding C. Thus, in a C-limited soil there appeared to be N available immediate for growth, while in an N-limited soil, easily available C was not immediately available. (c) 2014 Elsevier Ltd. All rights reserved.

AB - Application of C-rich plant residues can change the soil system from C-limitation for microbial growth to limitation by other nutrients. However, the initial nutrient status of the soil may interact with the added amount of residues in determining limitation. We studied this interactive effect in soils from the Harvard Forest LTER, where annual addition of N since 1988 has resulted in soils with different N-status: No N (Unfertilized), 50 (Low N) and 150 (High N) kg N ha(-1). We hypothesized that adding C-rich substrate would change the soil from being C- to being N-limited for bacterial growth and that the extent of Nlimitation would be higher with increasing substrate additions, while becoming less evident in soil with increasing N-status. We compared the effect of adding two C-rich substrates, starch (0, 10, 20, 40 mg g(-1) soil) and straw (0, 20, 40, 80 mg g(-1)), incubating the soils for up to 3 and 4 weeks for starch and straw, respectively. Nutrient limitations were studied by measuring bacterial growth 3 days after adding C as glucose and N as NH4NO3 in a full factorial design. Initially bacterial growth in all soils was C-limited. As hypothesized, adding C-rich substrates removed the C-limitation, with lower amounts of starch and straw needed in the unfertilized and Low N soils than in the High N soil. Combinations of different Nstatus of the soil and amendment levels of starch and straw could be found, where bacterial growth appeared close to co-limited both by available C and N. However, at even higher amendment levels, presumable resulting in N-limitation, bacterial growth still responded less by adding N then C-limited soils by adding C. Thus, in a C-limited soil there appeared to be N available immediate for growth, while in an N-limited soil, easily available C was not immediately available. (c) 2014 Elsevier Ltd. All rights reserved.

KW - N-deposition

KW - C-rich substrate

KW - Induced-N limitation

KW - Bacterial growth

KW - Leucine incorporation

KW - Limiting factors

U2 - 10.1016/j.soilbio.2014.02.015

DO - 10.1016/j.soilbio.2014.02.015

M3 - Article

VL - 74

SP - 11

EP - 20

JO - Soil Biology & Biochemistry

T2 - Soil Biology & Biochemistry

JF - Soil Biology & Biochemistry

SN - 0038-0717

ER -