Influence of bioreactor scale and complex medium on probing control of glucose feeding in cultivations of recombinant strains of Escherichia coli

Research output: Contribution to journalArticle


The objective of this work was to evaluate the performance of a feedback glucose control strategy (the probing strategy) in production relevant bioreactors with complex and mineral media. Experimental results from fedbatch cultivations with two recombinant Escherichia coli constructs expressing two different human therapeutic proteins were used to assess the performance and limitations of the glucose probing technique. Even though the performance of the probing strategy was affected by scale and complex media, this methodology rapidly identified a glucose feed protocol similar to an experimentally derived feed regime. This methodology may serve as a powerful tool for industrial process development and in optimization of glucose feed regimes when transferring process technology from one bioreactor system to another.


  • Stephane Velut
  • Andreas Castan
  • Kevin A. Short
  • Jan Peter Axelsson
  • Per Hagander
  • Barry A. Zditosky
  • Christopher W. Rysenga
  • Lena de Maré
  • Jan Haglund
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Control Engineering


  • probing control, feeding strategy, fed-batch, fermentations, industrial, bioreactor, complex medium
Original languageEnglish
Pages (from-to)816-824
JournalBiotechnology and Bioengineering
Issue number4
Publication statusPublished - 2007
Publication categoryResearch