Influence of twenty potentially antiviral substances on in vitro multiplication of hepatitis A virus

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Abstract

A multiwell tissue culture system was developed to study the influence of various substances on hepatitis A virus (HAV) propagation. A panel of 20 substances of different structure types, each with known effect against at least some viruses, was studied at a concentration of 100 microM. Three substances showed reproducible inhibition. The strongest inhibitor, arabinosylcytosine, also produced cytotoxic changes in cells down to a concentration of 1 microM, and its effect was considered as nonspecific. Amantadine and ribavirin showed a moderate effect at 100 microM. A stronger inhibition was seen at 250 and 500 microM, doses that are toxic and impractical for clinical use. Although no promising candidates for antiviral treatment of hepatitis A have emerged from the present study, the assay model described here would seem useful in the screening of substances with inhibitory effects on HAV.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Microbiology in the medical area

Keywords

  • ribavirin, amantadine, arabinosylcytosine, hepatitis A
Original languageEnglish
Pages (from-to)103-112
JournalAntiviral Research
Volume6
Issue number2
Publication statusPublished - 1986
Publication categoryResearch
Peer-reviewedYes