Influence of twenty potentially antiviral substances on in vitro multiplication of hepatitis A virus

Research output: Contribution to journalArticle


A multiwell tissue culture system was developed to study the influence of various substances on hepatitis A virus (HAV) propagation. A panel of 20 substances of different structure types, each with known effect against at least some viruses, was studied at a concentration of 100 microM. Three substances showed reproducible inhibition. The strongest inhibitor, arabinosylcytosine, also produced cytotoxic changes in cells down to a concentration of 1 microM, and its effect was considered as nonspecific. Amantadine and ribavirin showed a moderate effect at 100 microM. A stronger inhibition was seen at 250 and 500 microM, doses that are toxic and impractical for clinical use. Although no promising candidates for antiviral treatment of hepatitis A have emerged from the present study, the assay model described here would seem useful in the screening of substances with inhibitory effects on HAV.


Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Microbiology in the medical area


  • ribavirin, amantadine, arabinosylcytosine, hepatitis A
Original languageEnglish
Pages (from-to)103-112
JournalAntiviral Research
Issue number2
Publication statusPublished - 1986
Publication categoryResearch