Influence of twenty potentially antiviral substances on in vitro multiplication of hepatitis A virus

Research output: Contribution to journalArticle

Standard

Influence of twenty potentially antiviral substances on in vitro multiplication of hepatitis A virus. / Widell, Anders; Hansson, Bengt-Göran; Öberg, Bo; Nordenfelt, Erik.

In: Antiviral Research, Vol. 6, No. 2, 1986, p. 103-112.

Research output: Contribution to journalArticle

Harvard

APA

CBE

MLA

Vancouver

Author

Widell, Anders ; Hansson, Bengt-Göran ; Öberg, Bo ; Nordenfelt, Erik. / Influence of twenty potentially antiviral substances on in vitro multiplication of hepatitis A virus. In: Antiviral Research. 1986 ; Vol. 6, No. 2. pp. 103-112.

RIS

TY - JOUR

T1 - Influence of twenty potentially antiviral substances on in vitro multiplication of hepatitis A virus

AU - Widell, Anders

AU - Hansson, Bengt-Göran

AU - Öberg, Bo

AU - Nordenfelt, Erik

PY - 1986

Y1 - 1986

N2 - A multiwell tissue culture system was developed to study the influence of various substances on hepatitis A virus (HAV) propagation. A panel of 20 substances of different structure types, each with known effect against at least some viruses, was studied at a concentration of 100 microM. Three substances showed reproducible inhibition. The strongest inhibitor, arabinosylcytosine, also produced cytotoxic changes in cells down to a concentration of 1 microM, and its effect was considered as nonspecific. Amantadine and ribavirin showed a moderate effect at 100 microM. A stronger inhibition was seen at 250 and 500 microM, doses that are toxic and impractical for clinical use. Although no promising candidates for antiviral treatment of hepatitis A have emerged from the present study, the assay model described here would seem useful in the screening of substances with inhibitory effects on HAV.

AB - A multiwell tissue culture system was developed to study the influence of various substances on hepatitis A virus (HAV) propagation. A panel of 20 substances of different structure types, each with known effect against at least some viruses, was studied at a concentration of 100 microM. Three substances showed reproducible inhibition. The strongest inhibitor, arabinosylcytosine, also produced cytotoxic changes in cells down to a concentration of 1 microM, and its effect was considered as nonspecific. Amantadine and ribavirin showed a moderate effect at 100 microM. A stronger inhibition was seen at 250 and 500 microM, doses that are toxic and impractical for clinical use. Although no promising candidates for antiviral treatment of hepatitis A have emerged from the present study, the assay model described here would seem useful in the screening of substances with inhibitory effects on HAV.

KW - ribavirin

KW - amantadine

KW - arabinosylcytosine

KW - hepatitis A

U2 - 10.1016/0166-3542(86)90030-6

DO - 10.1016/0166-3542(86)90030-6

M3 - Article

VL - 6

SP - 103

EP - 112

JO - Antiviral Research

JF - Antiviral Research

SN - 0166-3542

IS - 2

ER -