Instantaneous isotropic volumetric imaging of fast biological processes
Research output: Contribution to journal › Article
To capture highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light fields yields three-dimensional images with high, isotropic spatial resolution and a significant reduction of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate medaka heart and blood flow imaging at single-cell resolution and free of motion artefacts at volume rates of up to 200 Hz.
|Research areas and keywords||
Subject classification (UKÄ) – MANDATORY
|Number of pages||4|
|Publication status||Published - 2019 Jun|