Instantaneous isotropic volumetric imaging of fast biological processes

Research output: Contribution to journalArticle


To capture highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light fields yields three-dimensional images with high, isotropic spatial resolution and a significant reduction of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate medaka heart and blood flow imaging at single-cell resolution and free of motion artefacts at volume rates of up to 200 Hz.


  • Nils Wagner
  • Nils Norlin
  • Jakob Gierten
  • Gustavo de Medeiros
  • Bálint Balázs
  • Joachim Wittbrodt
  • Lars Hufnagel
  • Robert Prevedel
External organisations
  • Karolinska Institutet
  • Friedrich Miescher Institute for Biomedical Research
  • University Hospital Heidelberg
  • Heidelberg University
  • European Molecular Biology Laboratory Heidelberg
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology


  • Algorithms, Animals, Animals, Genetically Modified, Artifacts, Blood Flow Velocity, Heart/diagnostic imaging, Humans, Image Processing, Computer-Assisted/methods, Imaging, Three-Dimensional/methods, Microscopy/methods, Oryzias, Single-Cell Analysis/methods
Original languageEnglish
Pages (from-to)497-500
Number of pages4
JournalNature Methods
Issue number6
Publication statusPublished - 2019 Jun
Publication categoryResearch
Externally publishedYes