Insulin stimulates ganglionic protein synthesis and reduces thymidine incorporation in support cells of the in vitro regenerating adult frog sciatic sensory neurons

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Abstract

Insulin was tested for effects on crush injured, in vitro regenerating, adult frog sciatic sensory axons. A wide range of insulin concentrations (0.01-10 μg × ml-1) was found to stimulate incorporation of radioactive leucine into ganglionic protein by 50-80%. without affecting the regeneration distance. Simultaneously insulin inhibited the proliferation of the support cells at the crush region by 30%, as measured by thymidine incorporation. Experiments using compartmentalized culture dishes indicated that the proliferation inhibitory effect could be indirect and mediated by the neuronal cells. The results suggest that insulin influences the metabolism of adult peripheral neuronal cell bodies. The stimulated nerve cells could in turn affect the proliferation of support cells in the nerve trunk.

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Subject classification (UKÄ) – MANDATORY

  • Cell Biology

Keywords

  • In vitro, Inhibition, Insulin, Nerve regeneration, Proliferation, Protein synthesis, Sciatic nerve, Stimulation
Original languageEnglish
Pages (from-to)183-186
JournalNeuroscience Letters
Volume132
Issue number2
Publication statusPublished - 1991 Nov 11
Publication categoryResearch
Peer-reviewedYes