Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples.

Research output: Contribution to journalArticle

Abstract

On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38-40%) rate was 50 μl min(-1) giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7-100 ng ml(-1) within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%.

Details

Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Clinical Medicine
  • Medicinal Chemistry
Original languageEnglish
Pages (from-to)1790-1796
JournalLab on a Chip
Volume13
Issue number9
Publication statusPublished - 2013
Publication categoryResearch
Peer-reviewedYes

Total downloads

No data available

Related research output

Klara Petersson, 2018 Feb 20, 1 ed. Lund: Department of Biomedical Engineering, Lund university. 159 p.

Research output: ThesisDoctoral Thesis (compilation)

View all (1)