Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples.

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Bibtex

@article{ce37e20365be437da2ed2684e1dd609d,
title = "Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples.",
abstract = "On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38-40{\%}) rate was 50 μl min(-1) giving a plasma volume fraction yield of ≈33{\%}. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7-100 ng ml(-1) within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13{\%}.",
author = "{Ahmad Tajudin}, Asilah and Klara Petersson and A Lenshof and A-M Sw{\"a}rd-Nilsson and Lena {\AA}berg and Gy{\"o}rgy Marko-Varga and Johan Malm and Hans Lilja and Thomas Laurell",
year = "2013",
doi = "10.1039/c3lc41269e",
language = "English",
volume = "13",
pages = "1790--1796",
journal = "Lab on a Chip - Miniaturisation for Chemistry and Biology",
issn = "1473-0189",
publisher = "Royal Society of Chemistry",
number = "9",

}