Integrated Brain Atlas for Unbiased Mapping of Nervous System Effects Following Liraglutide Treatment

Research output: Contribution to journalArticle

Abstract

Light Sheet Fluorescence Microscopy (LSFM) of whole organs, in particular the brain, offers a plethora of biological data imaged in 3D. This technique is however often hindered by cumbersome non-automated analysis methods. Here we describe an approach to fully automate the analysis by integrating with data from the Allen Institute of Brain Science (AIBS), to provide precise assessment of the distribution and action of peptide-based pharmaceuticals in the brain. To illustrate this approach, we examined the acute central nervous system effects of the glucagon-like peptide-1 (GLP-1) receptor agonist liraglutide. Peripherally administered liraglutide accessed the hypothalamus and brainstem, and led to activation in several brain regions of which most were intersected by projections from neurons in the lateral parabrachial nucleus. Collectively, we provide a rapid and unbiased analytical framework for LSFM data which enables quantification and exploration based on data from AIBS to support basic and translational discovery.

Details

Authors
  • Casper Bo Gravesen Salinas
  • Tess Tsai-Hsiu Lu
  • Sanaz Gabery
  • Kasper Marstal
  • Tomas Alanentalo
  • Aaron Jeffrey Mercer
  • Anda Cornea
  • Knut Conradsen
  • Jacob Hecksher-Sørensen
  • Anders Bjorholm Dahl
  • Lotte Bjerre Knudsen
  • Anna Secher
Organisations
External organisations
  • Technical University of Denmark
  • Novo Nordisk A/S
  • Erasmus University Medical Center
  • Novo Nordisk, Inc., US
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Bioinformatics (Computational Biology)
  • Neurosciences
Original languageEnglish
Article number10310
Number of pages12
JournalScientific Reports
Volume8
Issue number1
Publication statusPublished - 2018 Jul 9
Publication categoryResearch
Peer-reviewedYes