Integrated enzymatic reactions and analysis

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Abstract

Enzymatic reactions were performed in a modified auto-injector unit of a Shimadzu HPLC system. The reactions were analyzed by automated injections directly into the HPLC separation system. Two reactions were studied, and the enzymes mandelonitrile lyase and α-chymotrypsin were immobilized by adsorption onto a solid support, e.g., Celite and Chromosorb. The reactions were performed in various organic solvents e.g., diisopropyl ether, heptane/ethyl acetate mixtures and acetonitrile.

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Subject classification (UKÄ) – MANDATORY

  • Biocatalysis and Enzyme Technology
Original languageEnglish
Pages (from-to)165-168
Number of pages4
JournalBiotechnology Techniques
Volume6
Issue number2
Publication statusPublished - 1992 Mar 1
Publication categoryResearch
Peer-reviewedYes