Integrated flow-injection processing for on-line quantification of plasmid DNA during cultivation of E. coli

Research output: Contribution to journalArticle

Abstract

An integrated flow-injection processing (FIP) system for the quantification of plasmids during cultivation is described. The system performs on-line sampling, cell lysis, and quantification of plasmids in an integrated manner during cultivation of E. coli. The system was operated by using a miniaturized expanded-bed column which can be used for handling samples containing cells and cell debris without interfering with the binding analysis. Two types of detectors (one measuring UV absorbance at 254 nm and a fluorometer) are used for on-line plasmid detection. The system was developed using standard solutions and it was successfully applied in monitoring plasmid contents during a cultivation of E. coli.

Details

Authors
Organisations
External organisations
  • Lund University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Analytical Chemistry

Keywords

  • Escherichia coli, Flow-injection processing, On-line sampling, Plasmids
Original languageEnglish
Pages (from-to)406-411
Number of pages6
JournalBiotechnology and Bioengineering
Volume73
Issue number5
Publication statusPublished - 2001 Jun 5
Publication categoryResearch
Peer-reviewedYes