Integrated immobilized cell reactor–adsorption system for b-cyclodextrin production: a model study using PVA-cryogel entrapped Bacillus agaradhaerens cells.

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Abstract

Production of cyclodextrins (CDs) by immobilized cells of the alkaliphilic Bacillus agaradhaerens LS-3C with integrated product recovery was studied. The microorganism was entrapped in polyvinyl alcohol-cryogel beads and used as a convenient source of immobilized cyclodextrin glycosyltransferase (CGTase). On activation by incubation in the cultivation medium containing 1% (w/v) starch, the entrapped cells multiplied and secreted CGTase with an activity of 2–3 mg β-cyclodextrin h-1 g-1 beads. The immobilized biocatalyst exhibited maximum activity at pH 9 and 50 °C, and formed cyclodextrins comprising 92–94% β-CD and remaining α-CD. The cyclodextrin product from the immobilized cell bioreactor was continuously recovered by adsorption to Amberlite XAD-4 in a recycle batch mode. The product adsorption was facilitated at low temperature while hot water was used for elution.

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  • Industrial Biotechnology
Original languageEnglish
Pages (from-to)1537-1543
JournalBiotechnology Letters
Volume25
Issue number18
Publication statusPublished - 2003
Publication categoryResearch
Peer-reviewedYes