Integrated set up of sampling; sample treatment and assay of an intracellular protein during fermentation.

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Abstract

A complete system for on-line sampling, sample handling and analysis of fermentation processes has been set up. The system is designed such that a sample is taken from the fermentor, the metabolism of microbial cells is instantaneously arrested, the cells are lysed in order to release intracellular compounds and in a subsequent binding assay the content of target molecules is monitored. The system is constructed based on the flow-injection analysis concept. Good agreement between on-line analysis and off-line analysis is reported.

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  • Industrial Biotechnology
Original languageEnglish
Pages (from-to)366-371
JournalJournal of Biotechnology
Volume150
Issue number3
Publication statusPublished - 2010
Publication categoryResearch
Peer-reviewedYes