Interleukin-25 reduces Th17 cells and inflammatory responses in human peripheral blood mononuclear cells

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@article{c143869bd2294a7c929650b0d138bcac,
title = "Interleukin-25 reduces Th17 cells and inflammatory responses in human peripheral blood mononuclear cells",
abstract = "Background: The absence of interleukin-25 (IL-25) favors the induction of Th1 and Th17 immune responses in mice. Th1 immune responses have been associated with the pathology of atherosclerosis, a lipid and inflammation driven disease of the arterial wall. Purpose of research: To evaluate the effect of IL-25 on human peripheral blood mononuclear cells (hPBMCs) in the presence and absence of oxidized low density lipoprotein (oxLDL), a key player in atherosclerosis development. Principal results: Human PBMCs were incubated with recombinant human IL-25 (rhIL-25) in the presence and absence of oxLDL and analyzed with flow cytometry while cytokine secretion was measured in cell culture supernatants. The IL-25 receptor, IL-17RB, was mostly expressed on T cells. Incubation of hPBMCs with IL-25 reduced the frequency of Th17 cells. Furthermore, IL-25 inhibited the release of the Th17-inducing cytokine IL-6 from dendritic cells isolated from hPBMCs indicating that the IL-25 mediated Th17 suppression may be indirect. Moreover, IL-25 reduced the secretion of the proinflammatory cytokine IFNγ from hPBMCs. OxLDL decreased IFNγ release from hPBMCs regardless of the presence or absence of IL-25. Conclusions: IL-25 reduces Th1 and Th17 immune responses in hPBMCs raising the interesting possibility that IL-25 could have a protective role in human atherosclerosis.",
keywords = "IL-25, Oxidized LDL, Th17",
author = "Mantani, {Polyxeni T.} and Jenifer Vallejo and Irena Ljungcrantz and Jan Nilsson and Harry Bj{\"o}rkbacka and Fredrikson, {Gunilla Nordin}",
year = "2018",
doi = "10.1016/j.humimm.2018.06.008",
language = "English",
volume = "79",
pages = "685--692",
journal = "Human Immunology",
issn = "0198-8859",
publisher = "Elsevier",
number = "9",

}