Interleukin-25 reduces Th17 cells and inflammatory responses in human peripheral blood mononuclear cells

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Interleukin-25 reduces Th17 cells and inflammatory responses in human peripheral blood mononuclear cells. / Mantani, Polyxeni T.; Vallejo, Jenifer; Ljungcrantz, Irena; Nilsson, Jan; Björkbacka, Harry; Fredrikson, Gunilla Nordin.

In: Human Immunology, Vol. 79, No. 9, 2018, p. 685-692.

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T1 - Interleukin-25 reduces Th17 cells and inflammatory responses in human peripheral blood mononuclear cells

AU - Mantani, Polyxeni T.

AU - Vallejo, Jenifer

AU - Ljungcrantz, Irena

AU - Nilsson, Jan

AU - Björkbacka, Harry

AU - Fredrikson, Gunilla Nordin

PY - 2018

Y1 - 2018

N2 - Background: The absence of interleukin-25 (IL-25) favors the induction of Th1 and Th17 immune responses in mice. Th1 immune responses have been associated with the pathology of atherosclerosis, a lipid and inflammation driven disease of the arterial wall. Purpose of research: To evaluate the effect of IL-25 on human peripheral blood mononuclear cells (hPBMCs) in the presence and absence of oxidized low density lipoprotein (oxLDL), a key player in atherosclerosis development. Principal results: Human PBMCs were incubated with recombinant human IL-25 (rhIL-25) in the presence and absence of oxLDL and analyzed with flow cytometry while cytokine secretion was measured in cell culture supernatants. The IL-25 receptor, IL-17RB, was mostly expressed on T cells. Incubation of hPBMCs with IL-25 reduced the frequency of Th17 cells. Furthermore, IL-25 inhibited the release of the Th17-inducing cytokine IL-6 from dendritic cells isolated from hPBMCs indicating that the IL-25 mediated Th17 suppression may be indirect. Moreover, IL-25 reduced the secretion of the proinflammatory cytokine IFNγ from hPBMCs. OxLDL decreased IFNγ release from hPBMCs regardless of the presence or absence of IL-25. Conclusions: IL-25 reduces Th1 and Th17 immune responses in hPBMCs raising the interesting possibility that IL-25 could have a protective role in human atherosclerosis.

AB - Background: The absence of interleukin-25 (IL-25) favors the induction of Th1 and Th17 immune responses in mice. Th1 immune responses have been associated with the pathology of atherosclerosis, a lipid and inflammation driven disease of the arterial wall. Purpose of research: To evaluate the effect of IL-25 on human peripheral blood mononuclear cells (hPBMCs) in the presence and absence of oxidized low density lipoprotein (oxLDL), a key player in atherosclerosis development. Principal results: Human PBMCs were incubated with recombinant human IL-25 (rhIL-25) in the presence and absence of oxLDL and analyzed with flow cytometry while cytokine secretion was measured in cell culture supernatants. The IL-25 receptor, IL-17RB, was mostly expressed on T cells. Incubation of hPBMCs with IL-25 reduced the frequency of Th17 cells. Furthermore, IL-25 inhibited the release of the Th17-inducing cytokine IL-6 from dendritic cells isolated from hPBMCs indicating that the IL-25 mediated Th17 suppression may be indirect. Moreover, IL-25 reduced the secretion of the proinflammatory cytokine IFNγ from hPBMCs. OxLDL decreased IFNγ release from hPBMCs regardless of the presence or absence of IL-25. Conclusions: IL-25 reduces Th1 and Th17 immune responses in hPBMCs raising the interesting possibility that IL-25 could have a protective role in human atherosclerosis.

KW - IL-25

KW - Oxidized LDL

KW - Th17

UR - http://www.scopus.com/inward/record.url?scp=85049309749&partnerID=8YFLogxK

U2 - 10.1016/j.humimm.2018.06.008

DO - 10.1016/j.humimm.2018.06.008

M3 - Article

VL - 79

SP - 685

EP - 692

JO - Human Immunology

T2 - Human Immunology

JF - Human Immunology

SN - 0198-8859

IS - 9

ER -