Intestinal preservation injury: A comparison between rat, porcine and human intestines

Research output: Contribution to journalArticle

Abstract

Advanced preservation injury (PI) after intestinal transplantation has deleterious short-and long-term effects and constitutes a major research topic. Logistics and costs favor rodent studies, whereas clinical translation mandates studies in larger animals or using human material. Despite diverging reports, no direct comparison between the development of intestinal PI in rats, pigs, and humans is available. We compared the development of PI in rat, porcine, and human intestines. Intestinal procurement and cold storage (CS) using histidine–tryptophan–ketoglutarate solution was performed in rats, pigs, and humans. Tissue samples were obtained after 8, 14, and 24 h of CS), and PI was assessed morphologically and at the molecular level (cleaved caspase-3, zonula occludens, claudin-3 and 4, tricellulin, occludin, cytokeratin-8) using immunohistochemistry and Western blot. Intestinal PI developed slower in pigs compared to rats and humans. Tissue injury and apoptosis were significantly higher in rats. Tight junction proteins showed quantitative and qualitative changes differing between species. Significant interspecies differences exist between rats, pigs, and humans regarding intestinal PI progression at tissue and molecular levels. These differences should be taken into account both with regards to study design and the interpretation of findings when relating them to the clinical setting.

Details

Authors
  • John Mackay Søfteland
  • Anna Casselbrant
  • Ali Reza Biglarnia
  • Johan Linders
  • Mats Hellström
  • Antonio Pesce
  • Arvind Manikantan Padma
  • Lucian Petru Jiga
  • Bogdan Hoinoiu
  • Mihai Ionac
  • Mihai Oltean
External organisations
  • Sahlgrenska University Hospital
  • Sahlgrenska Academy
  • Skåne University Hospital
  • University of Catania
  • Victor Babes University of Medicine and Pharmacy
  • Evangelisches Krankenhaus Oldenburg
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Pharmacology and Toxicology

Keywords

  • Intestinal mucosa, Intestine, Ischemia, Organ preservation, Tight junctions, Transplantation
Original languageEnglish
Article number3135
JournalInternational Journal of Molecular Sciences
Volume20
Issue number13
Publication statusPublished - 2019 Jul 1
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes