Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering

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Bibtex

@article{35ac359dbd094b43a41baf57403f3534,
title = "Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering",
abstract = "A novel and generally applicable method for determining structures of membrane proteins in solution via small-angle neutron scattering (SANS) is presented. Common detergents for solubilizing membrane proteins were synthesized in isotope-substituted versions for utilizing the intrinsic neutron scattering length difference between hydrogen and deuterium. Individual hydrogen/deuterium levels of the detergent head and tail groups were achieved such that the formed micelles became effectively invisible in heavy water (D2 O) when investigated by neutrons. This way, only the signal from the membrane protein remained in the SANS data. We demonstrate that the method is not only generally applicable on five very different membrane proteins but also reveals subtle structural details about the sarco/endoplasmatic reticulum Ca2+ ATPase (SERCA). In all, the synthesis of isotope-substituted detergents makes solution structure determination of membrane proteins by SANS and subsequent data analysis available to non-specialists. This article is protected by copyright. All rights reserved.",
author = "Midtgaard, {S{\o}ren Roi} and Darwish, {Tamim A} and Pedersen, {Martin Cramer} and Pie Huda and Larsen, {Andreas Haahr} and Jensen, {Grethe Vestergaard} and Kynde, {S{\o}ren Andreas R{\o}ssel} and Nicholas Skar-Gislinge and Nielsen, {Agnieszka Janina Zygadlo} and Claus Olesen and Mickael Blaise and Dorosz, {Jerzy J{\'o}zef} and Thorsen, {Thor Seneca} and Raminta Venskutonytė and Christian Krintel and M{\o}ller, {Jesper V} and Henrich Frielinghaus and Gilbert, {Elliot Paul} and Anne Martel and Kastrup, {Jette Sandholm} and Jensen, {Poul Erik} and Poul Nissen and Lise Arleth",
note = "This article is protected by copyright. All rights reserved.",
year = "2017",
month = "11",
day = "27",
doi = "10.1111/febs.14345",
language = "English",
journal = "The FEBS Journal",
issn = "1742-464X",
publisher = "Federation of European Neuroscience Societies and Blackwell Publishing Ltd",

}