Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering

Research output: Contribution to journalArticle

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Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering. / Midtgaard, Søren Roi; Darwish, Tamim A; Pedersen, Martin Cramer; Huda, Pie; Larsen, Andreas Haahr; Jensen, Grethe Vestergaard; Kynde, Søren Andreas Røssel; Skar-Gislinge, Nicholas; Nielsen, Agnieszka Janina Zygadlo; Olesen, Claus; Blaise, Mickael; Dorosz, Jerzy Józef; Thorsen, Thor Seneca; Venskutonytė, Raminta; Krintel, Christian; Møller, Jesper V; Frielinghaus, Henrich; Gilbert, Elliot Paul; Martel, Anne; Kastrup, Jette Sandholm; Jensen, Poul Erik; Nissen, Poul; Arleth, Lise.

In: The FEBS Journal, 27.11.2017.

Research output: Contribution to journalArticle

Harvard

Midtgaard, SR, Darwish, TA, Pedersen, MC, Huda, P, Larsen, AH, Jensen, GV, Kynde, SAR, Skar-Gislinge, N, Nielsen, AJZ, Olesen, C, Blaise, M, Dorosz, JJ, Thorsen, TS, Venskutonytė, R, Krintel, C, Møller, JV, Frielinghaus, H, Gilbert, EP, Martel, A, Kastrup, JS, Jensen, PE, Nissen, P & Arleth, L 2017, 'Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering', The FEBS Journal. https://doi.org/10.1111/febs.14345

APA

Midtgaard, S. R., Darwish, T. A., Pedersen, M. C., Huda, P., Larsen, A. H., Jensen, G. V., ... Arleth, L. (2017). Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering. The FEBS Journal. https://doi.org/10.1111/febs.14345

CBE

Midtgaard SR, Darwish TA, Pedersen MC, Huda P, Larsen AH, Jensen GV, Kynde SAR, Skar-Gislinge N, Nielsen AJZ, Olesen C, Blaise M, Dorosz JJ, Thorsen TS, Venskutonytė R, Krintel C, Møller JV, Frielinghaus H, Gilbert EP, Martel A, Kastrup JS, Jensen PE, Nissen P, Arleth L. 2017. Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering. The FEBS Journal. https://doi.org/10.1111/febs.14345

MLA

Vancouver

Author

Midtgaard, Søren Roi ; Darwish, Tamim A ; Pedersen, Martin Cramer ; Huda, Pie ; Larsen, Andreas Haahr ; Jensen, Grethe Vestergaard ; Kynde, Søren Andreas Røssel ; Skar-Gislinge, Nicholas ; Nielsen, Agnieszka Janina Zygadlo ; Olesen, Claus ; Blaise, Mickael ; Dorosz, Jerzy Józef ; Thorsen, Thor Seneca ; Venskutonytė, Raminta ; Krintel, Christian ; Møller, Jesper V ; Frielinghaus, Henrich ; Gilbert, Elliot Paul ; Martel, Anne ; Kastrup, Jette Sandholm ; Jensen, Poul Erik ; Nissen, Poul ; Arleth, Lise. / Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering. In: The FEBS Journal. 2017.

RIS

TY - JOUR

T1 - Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering

AU - Midtgaard, Søren Roi

AU - Darwish, Tamim A

AU - Pedersen, Martin Cramer

AU - Huda, Pie

AU - Larsen, Andreas Haahr

AU - Jensen, Grethe Vestergaard

AU - Kynde, Søren Andreas Røssel

AU - Skar-Gislinge, Nicholas

AU - Nielsen, Agnieszka Janina Zygadlo

AU - Olesen, Claus

AU - Blaise, Mickael

AU - Dorosz, Jerzy Józef

AU - Thorsen, Thor Seneca

AU - Venskutonytė, Raminta

AU - Krintel, Christian

AU - Møller, Jesper V

AU - Frielinghaus, Henrich

AU - Gilbert, Elliot Paul

AU - Martel, Anne

AU - Kastrup, Jette Sandholm

AU - Jensen, Poul Erik

AU - Nissen, Poul

AU - Arleth, Lise

N1 - This article is protected by copyright. All rights reserved.

PY - 2017/11/27

Y1 - 2017/11/27

N2 - A novel and generally applicable method for determining structures of membrane proteins in solution via small-angle neutron scattering (SANS) is presented. Common detergents for solubilizing membrane proteins were synthesized in isotope-substituted versions for utilizing the intrinsic neutron scattering length difference between hydrogen and deuterium. Individual hydrogen/deuterium levels of the detergent head and tail groups were achieved such that the formed micelles became effectively invisible in heavy water (D2 O) when investigated by neutrons. This way, only the signal from the membrane protein remained in the SANS data. We demonstrate that the method is not only generally applicable on five very different membrane proteins but also reveals subtle structural details about the sarco/endoplasmatic reticulum Ca2+ ATPase (SERCA). In all, the synthesis of isotope-substituted detergents makes solution structure determination of membrane proteins by SANS and subsequent data analysis available to non-specialists. This article is protected by copyright. All rights reserved.

AB - A novel and generally applicable method for determining structures of membrane proteins in solution via small-angle neutron scattering (SANS) is presented. Common detergents for solubilizing membrane proteins were synthesized in isotope-substituted versions for utilizing the intrinsic neutron scattering length difference between hydrogen and deuterium. Individual hydrogen/deuterium levels of the detergent head and tail groups were achieved such that the formed micelles became effectively invisible in heavy water (D2 O) when investigated by neutrons. This way, only the signal from the membrane protein remained in the SANS data. We demonstrate that the method is not only generally applicable on five very different membrane proteins but also reveals subtle structural details about the sarco/endoplasmatic reticulum Ca2+ ATPase (SERCA). In all, the synthesis of isotope-substituted detergents makes solution structure determination of membrane proteins by SANS and subsequent data analysis available to non-specialists. This article is protected by copyright. All rights reserved.

U2 - 10.1111/febs.14345

DO - 10.1111/febs.14345

M3 - Article

JO - The FEBS Journal

JF - The FEBS Journal

SN - 1742-464X

ER -