Involvement of Rac GTPase activation in phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to ICAM-1

Research output: Contribution to journalArticle

Abstract

Increasing evidence indicates that phospholipid oxidation plays important roles in atherosclerosis. Here, we investigated the involvement of Rho-family GTPases inphosphatidylcholine hydroperoxide (PCOOH)-induced THP-1 cell adhesion to ICAM-1. Isoprenoid depletion by fluvastatin and geranylgeranyltransferase inhibition by GGTI-286 suppressed PCOOH-induced cell adhesion to ICAM-1 and F-actin-rich membrane protrusion formation. Pull-down assays demonstrated the activation of Rac1 and Rac2 in PCOOH-treated cells. Pan-Rho-family GTPase inhibitor Clostridium difficile toxin B, Rac-specific inhibitor NSC23776, and RNA interference of the Rac isoforms suppressed the cell adhesion. These findings indicate the involvement of Rac GTPase activation in PCOOH-induced cell adhesion to ICAM-1 via actin reorganization.

Details

Authors
  • Akira Asai
  • Fumitaka Okajima
  • Yasushi Nakajima
  • Mototsugu Nagao
  • Kiyotaka Nakagawa
  • Teruo Miyazawa
  • Shinichi Oikawa
External organisations
  • Nippon Medical School
  • Tohoku University
Research areas and keywords

Keywords

  • Actin, Cell adhesion, Monocyte, Phosphatidylcholine hydroperoxide, Phospholipid oxidation, Rac
Original languageEnglish
Pages (from-to)273-277
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume406
Issue number2
Publication statusPublished - 2011 Mar 11
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes