IRF8 Transcription-Factor-Dependent Classical Dendritic Cells Are Essential for Intestinal T Cell Homeostasis

Research output: Contribution to journalArticle

Abstract

The role of dendritic cells (DCs) in intestinal immune homeostasis remains incompletely defined. Here we show that mice lacking IRF8 transcription-factor-dependent DCs had reduced numbers of T cells in the small intestine (SI), but not large intestine (LI), including an almost complete absence of SI CD8αβ+ and CD4+CD8αα+ T cells; the latter requiring β8 integrin expression by migratory IRF8 dependent CD103+CD11b- DCs. SI homing receptor induction was impaired during T cell priming in mesenteric lymph nodes (MLN), which correlated with a reduction in aldehyde dehydrogenase activity by SI-derived MLN DCs, and inefficient T cell localization to the SI. These mice also lacked intestinal T helper 1 (Th1) cells, and failed to support Th1 cell differentiation in MLN and mount Th1 cell responses to Trichuris muris infection. Collectively these results highlight multiple non-redundant roles for IRF8 dependent DCs in the maintenance of intestinal T cell homeostasis.

Details

Authors
Organisations
External organisations
  • Technical University of Denmark
  • Ghent University
  • University of Copenhagen
  • University of Manchester
  • Oslo university hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology
Original languageEnglish
Pages (from-to)860-874
Number of pages15
JournalImmunity
Volume44
Issue number4
Publication statusPublished - 2016 Apr 19
Publication categoryResearch
Peer-reviewedYes

Related research output

Müller Luda, K., 2018, Lund: Lund University, Faculty of Medicine. 97 p.

Research output: ThesisDoctoral Thesis (monograph)

Demiri, M., 2017, Lund : Lund University, Faculty of Medicine. 69 p.

Research output: ThesisDoctoral Thesis (compilation)

View all (2)