Is cartilage sGAG content related to early changes in cartilage disease? Implications for interpretation of dGEMRIC.

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Is cartilage sGAG content related to early changes in cartilage disease? Implications for interpretation of dGEMRIC. / Stubendorff, Johann; Lammentausta, Eveliina; Struglics, André; Lindberg, Lisbeth; Heinegård, Dick; Dahlberg, Leif.

In: Osteoarthritis and Cartilage, Vol. 20, No. 5, 2012, p. 396-404.

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Stubendorff, Johann ; Lammentausta, Eveliina ; Struglics, André ; Lindberg, Lisbeth ; Heinegård, Dick ; Dahlberg, Leif. / Is cartilage sGAG content related to early changes in cartilage disease? Implications for interpretation of dGEMRIC. In: Osteoarthritis and Cartilage. 2012 ; Vol. 20, No. 5. pp. 396-404.

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TY - JOUR

T1 - Is cartilage sGAG content related to early changes in cartilage disease? Implications for interpretation of dGEMRIC.

AU - Stubendorff, Johann

AU - Lammentausta, Eveliina

AU - Struglics, André

AU - Lindberg, Lisbeth

AU - Heinegård, Dick

AU - Dahlberg, Leif

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Joint and Soft Tissue Unit (013242920), Connective Tissue Biology (013230151), Clinical and Molecular Osteoporosis Research Unit (013242930), Department of Orthopaedics (Lund) (013028000)

PY - 2012

Y1 - 2012

N2 - OBJECTIVE: This study investigates sulphated glycosaminoglycans (sGAG) content changes in early osteoarthritis (OA), and whether contrast-enhanced magnetic resonance imaging (MRI) of cartilage in vitro may identify early event of OA pathology. METHOD: Osteochondral plugs from patients with hip OA or femoral neck fracture (reference group) were collected and analysed by 1.5 T MRI with ΔR1 as a measure of cartilage contrast concentration. Cartilage hydration, contents of sGAG, cartilage oligomeric matrix protein (COMP), hydroxyproline, denatured collagen, and aggrecan TEGE(392) neoepitope were determined and histological grading was performed. RESULTS: sGAG content correlated to ΔR1, although no difference in either of these parameters was detectable between OA and reference cartilage at 4 h of contrast equilibration. In contrast, biochemical analysis of other cartilage matrix constituents showed distinct alterations typical for early cartilage degradation in OA cartilage and with clear evidence for increased aggrecan turnover. CONCLUSION: In the present in vitro study, cartilage sGAG content could not distinguish between early OA cartilage and reference cartilage. Given, that delayed gadolinium enhanced MRI of cartilage (dGEMRIC) indicates early events in the pathogenesis of OA in vivo, our results from the in vitro studies imply other, additional factors than cartilage sGAG content, e.g., alterations in diffusion or increased supply of contrast agent in the diseased joint. Alternatively, an altered dGEMRIC reflects later stages of OA, when sGAG content decreases. Further investigations are warranted, to understand variations in sGAG content in pathology, an essential background for interpreting dGEMRIC measurements.

AB - OBJECTIVE: This study investigates sulphated glycosaminoglycans (sGAG) content changes in early osteoarthritis (OA), and whether contrast-enhanced magnetic resonance imaging (MRI) of cartilage in vitro may identify early event of OA pathology. METHOD: Osteochondral plugs from patients with hip OA or femoral neck fracture (reference group) were collected and analysed by 1.5 T MRI with ΔR1 as a measure of cartilage contrast concentration. Cartilage hydration, contents of sGAG, cartilage oligomeric matrix protein (COMP), hydroxyproline, denatured collagen, and aggrecan TEGE(392) neoepitope were determined and histological grading was performed. RESULTS: sGAG content correlated to ΔR1, although no difference in either of these parameters was detectable between OA and reference cartilage at 4 h of contrast equilibration. In contrast, biochemical analysis of other cartilage matrix constituents showed distinct alterations typical for early cartilage degradation in OA cartilage and with clear evidence for increased aggrecan turnover. CONCLUSION: In the present in vitro study, cartilage sGAG content could not distinguish between early OA cartilage and reference cartilage. Given, that delayed gadolinium enhanced MRI of cartilage (dGEMRIC) indicates early events in the pathogenesis of OA in vivo, our results from the in vitro studies imply other, additional factors than cartilage sGAG content, e.g., alterations in diffusion or increased supply of contrast agent in the diseased joint. Alternatively, an altered dGEMRIC reflects later stages of OA, when sGAG content decreases. Further investigations are warranted, to understand variations in sGAG content in pathology, an essential background for interpreting dGEMRIC measurements.

U2 - 10.1016/j.joca.2012.01.015

DO - 10.1016/j.joca.2012.01.015

M3 - Article

VL - 20

SP - 396

EP - 404

JO - Osteoarthritis and Cartilage

T2 - Osteoarthritis and Cartilage

JF - Osteoarthritis and Cartilage

SN - 1063-4584

IS - 5

ER -