Isolation and characterization of primary bone marrow mesenchymal stromal cells

Research output: Contribution to journalArticle

Abstract

Bone marrow (BM) contains a rare population of mesenchymal stromal cells (MSCs), which have been characterized as nonhematopoietic skeletal progenitor cells with central importance for the hematopoietic microenvironment. Classically, MSCs are isolated by plastic adherence and subsequent culture. However, as cultured stromal cells differ from their in vivo progenitors, it is important to identify the phenotype of the primary MSCs to study these cells in more detail. In the past years, several surface markers have been reported to be suitable for effective enrichment of BM-MSCs, and recent data indicate that the putative MSC stem/progenitor cell population in human adult BM is highly enriched in Lin CD45 CD271+ CD140a (PDGFRα)low/− cells. Moreover, surface marker combinations have been described for the isolation of MSCs from murine BM. On the basis of these findings, the role of primary MSCs can now be studied in normal and, importantly, diseased BM. Furthermore, genetically engineered mouse models have been developed as powerful tools to investigate well-defined BM stromal cell populations in vivo. Our discussion aims to provide a concise overview of the current state of the art in BM-MSC isolation in humans and briefly present murine MSC isolation approaches and genetic models.

Details

Authors
Organisations
External organisations
  • Skåne University Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medical Genetics

Keywords

  • bone marrow, bone marrow stromal cells, CD271, CFU-F, mesenchymal stromal cells, MSC, PDGFRα
Original languageEnglish
Pages (from-to)109-118
Number of pages10
JournalAnnals of the New York Academy of Sciences
Volume1370
Issue number1
Publication statusPublished - 2016 Apr 1
Publication categoryResearch
Peer-reviewedYes