Isolation and identification from Salvia officinalis of two diterpenes which inhibit t-butylbicyclophosphoro[35S]thionate binding to chloride channel of rat cerebrocortical membranes in vitro

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@article{8c26a01a40044fb3bbe189bf91155ca7,
title = "Isolation and identification from Salvia officinalis of two diterpenes which inhibit t-butylbicyclophosphoro[35S]thionate binding to chloride channel of rat cerebrocortical membranes in vitro",
abstract = "Ethanolic extracts from dried leaves of sage (Salvia officinalis) showed inhibition of [35S]tertiary-butylbicyclophosphorothionate ([35S]TBPS) binding to rat brain membranes in vitro. This ligand is considered to bind to the chloride channel of the GABA/benzodiazepine receptor complex in brain tissue. Substances having inhibitory activity were purified and their chemical structure identified as the diterpenes carnosic acid and carnosol (IC50 values of 33 +/- 3 microM and 57 +/- 4 microM, respectively). The two compounds did not affect binding of the ligands [3H]muscimol and [3H]diazepam to the GABA/benzodiazepine complex in vitro. Saturation experiments of [35S]TBPS binding indicated that carnosic acid decreases the binding affinity.",
keywords = "γ-Aminobutyric acid, Benzodiazepine receptor, [35S]TBPS binding, Salvia officinalis, Diterpenoid, Carnosol, Carnosic acid",
author = "Rutherford, {Diane M} and Nielsen, {Mogens P} and Hansen, {Susanne K} and Michael-Robin Witt and Ola Bergendorff and Olov Sterner",
note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Dermatology and Venereology (Lund) (013006000), Organic chemistry (S/LTH) (011001240)",
year = "1992",
doi = "10.1016/0304-3940(92)90441-9",
language = "English",
volume = "135",
pages = "224--226",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier",
number = "2",

}