Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation

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Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation. / Lundwall, Åke; Dackowski, W.; Cohen, E.; Shaffer, M.; Mahr, A.; Dahlback, B.; Stenflo, J.; Wydro, R.

In: Proc Natl Acad Sci U S A, Vol. 83, No. 18, 1986, p. 6716-20.

Research output: Contribution to journalArticle

Harvard

Lundwall, Å, Dackowski, W, Cohen, E, Shaffer, M, Mahr, A, Dahlback, B, Stenflo, J & Wydro, R 1986, 'Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation', Proc Natl Acad Sci U S A, vol. 83, no. 18, pp. 6716-20.

APA

Lundwall, Å., Dackowski, W., Cohen, E., Shaffer, M., Mahr, A., Dahlback, B., ... Wydro, R. (1986). Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation. Proc Natl Acad Sci U S A, 83(18), 6716-20.

CBE

Lundwall Å, Dackowski W, Cohen E, Shaffer M, Mahr A, Dahlback B, Stenflo J, Wydro R. 1986. Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation. Proc Natl Acad Sci U S A. 83(18):6716-20.

MLA

Vancouver

Lundwall Å, Dackowski W, Cohen E, Shaffer M, Mahr A, Dahlback B et al. Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation. Proc Natl Acad Sci U S A. 1986;83(18):6716-20.

Author

Lundwall, Åke ; Dackowski, W. ; Cohen, E. ; Shaffer, M. ; Mahr, A. ; Dahlback, B. ; Stenflo, J. ; Wydro, R. / Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation. In: Proc Natl Acad Sci U S A. 1986 ; Vol. 83, No. 18. pp. 6716-20.

RIS

TY - JOUR

T1 - Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation

AU - Lundwall, Åke

AU - Dackowski, W.

AU - Cohen, E.

AU - Shaffer, M.

AU - Mahr, A.

AU - Dahlback, B.

AU - Stenflo, J.

AU - Wydro, R.

N1 - 18

PY - 1986

Y1 - 1986

N2 - Protein S is a cofactor of activated protein C; together they function as a regulator of blood coagulation. A human liver cDNA library constructed in bacteriophage lambda gt11 was screened with DNA fragments from a full-length bovine cDNA clone encoding protein S. Several cDNA clones were isolated and sequenced. The combined cDNA sequences encoded the mature protein and 15 residues of the leader sequence when compared to bovine protein S. Human protein S is a single-chain protein consisting of 635 amino acids with 82% homology to bovine protein S. After an NH2-terminal gamma-carboxyglutamic acid-containing region, there is a short region with thrombin-sensitive bond(s), followed by a region with four repeat sequences that are homologous to the precursor of mouse epidermal growth factor. In contrast to the other vitamin K-dependent plasma proteins, the COOH-terminal portion of human protein S does not show any resemblance to serine proteases.

AB - Protein S is a cofactor of activated protein C; together they function as a regulator of blood coagulation. A human liver cDNA library constructed in bacteriophage lambda gt11 was screened with DNA fragments from a full-length bovine cDNA clone encoding protein S. Several cDNA clones were isolated and sequenced. The combined cDNA sequences encoded the mature protein and 15 residues of the leader sequence when compared to bovine protein S. Human protein S is a single-chain protein consisting of 635 amino acids with 82% homology to bovine protein S. After an NH2-terminal gamma-carboxyglutamic acid-containing region, there is a short region with thrombin-sensitive bond(s), followed by a region with four repeat sequences that are homologous to the precursor of mouse epidermal growth factor. In contrast to the other vitamin K-dependent plasma proteins, the COOH-terminal portion of human protein S does not show any resemblance to serine proteases.

KW - Protein S

KW - Humans

KW - Glycoproteins/analysis/genetics

KW - Epidermal Growth Factor/analysis

KW - DNA/analysis/isolation & purification

KW - Amino Acid Sequence

KW - Base Sequence

KW - RNA

KW - Messenger/analysis

KW - Research Support

KW - Non-U.S. Gov't

M3 - Article

VL - 83

SP - 6716

EP - 6720

JO - Proc Natl Acad Sci U S A

JF - Proc Natl Acad Sci U S A

IS - 18

ER -